A rapid, simple, and economical method for the isolation of ribosomes and translational machinery for structural and functional studies.

ABSTRACT: Ribosomes are RNA-protein complexes essential for protein synthesis and quality control. Traditional methods for ribosome isolation are labor-intensive, expensive, and require a substantial amount of biological material. In contrast, our method, RNA affinity purification using poly-lysine (RAPPL), provides a rapid, simple, and cost-effective alternative applicable to various species and types of starting material (cell lysates, whole cells, organs, or whole organisms). It is also compatible with traditional isolation techniques. Here, we describe the use of RAPPL for rapid isolation, functional screening, and structural analysis of ribosomes and associated factors. We also demonstrate the application of RAPPL in investigating ribosome-associated resistance mechanisms in uropathogenic Esche

SUBMITTER: Erath J 

PROVIDER: S-EPMC12325604 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature