Integrating network pharmacology and experimental validation to explore the mechanism of Yiqi Ziyin in treating immune thrombocytopenia.

ABSTRACT: To investigate the mechanism and potential targets of Yiqi Ziyin (YQZY) for treating immune thrombocytopenia (ITP). Prednisone and YQZY were orally administered to ITP mice. Post-treatment, blood samples were taken to evaluate the blood picture. The spleen was obtained to observe histomorphological changes and calculate the spleen index. The active components and predicted related targets of YQZY were obtained based on the TCMSP database. The ITP-related targets were screened by GeneCards and DisGeNET databases. Then, functional enrichment analyses were conducted to examine the potential regulatory pathways of YQZY in ITP, which was validated by western blot assay. Additionally, three different networks were constructed to identify the predominant targets and ingredients of YQZY in the ITP

SUBMITTER: Wang J 

PROVIDER: S-EPMC12325705 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature