Comparative proteomics analysis of MRSA under different experimental conditions.

ABSTRACT: The ability of methicillin-resistant Staphylococcus aureus (MRSA) to adapt to environmental stressors is crucial for its survival and persistence. This study used shotgun proteomics to analyze the protein profiles of MRSA strains ST398 and JE2 under three experimental conditions: EC1 (control, 37 °C, pH 7), EC2 (35 °C, pH 6), and EC3 (35 °C, pH 6 with 5% NaCl). Proteins were extracted and digested with trypsin using filter-aided sample preparation (FASP), followed by LC-MS/MS analysis. Raw data were analyzed using Proteome Discoverer software with label-free quantification (LFQ), identifying an average of 2640 ± 70 and 2832 ± 154 proteins in ST398 and JE2, respectively. Qualitative and quantitative differences in protein expression were observed between strains and conditions. Under EC2, A

SUBMITTER: Al Nahhas D 

PROVIDER: S-EPMC12325769 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature