Bridging the marrow: a co-culture-platform of leukemia cells and MS5-derived stromal cells or adipocytes.

ABSTRACT: In the context of acute lymphoblastic leukemia (ALL), the bone marrow microenvironment (BMM) plays a crucial role in providing pro-survival signals, as evident from the rapid spontaneous cell death observed in ex-vivo cultures of primary ALL cells. Among the diverse cell types within the BMM, bone marrow adipocytes (BMAd) exhibit significant plasticity and can constitute a substantial part of the BMM, especially during ALL therapy. However, conflicting data on the interaction between ALL cells and adipocytes have been reported, potentially arising from variations in culture systems and conditions. Our study aimed to establish a chemically defined co-culture system of leukemia cells combined with either bone marrow (BM)-derived stromal cells or adipocytes. Established B-precursor ALL cell l

SUBMITTER: Zinngrebe J 

PROVIDER: S-EPMC12325993 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature