The Challenges and Opportunities of High-Spin Mn(II) EPR and ENDOR.

ABSTRACT: We examine the Electron Paramagnetic Resonance (EPR) and Electron-Nuclear Double Resonance (ENDOR) spectroscopy of three quite distinct high-spin Mn(II) systems and describe experimental techniques and methods of analysis that are useful in their study. We demonstrate that this S=5/2 metal center provides useful orientation-selection through the Zero-Field Splitting (ZFS) tensor that enables determination of a ¹³C hyperfine-coupling tensor with extremely small hyperfine interaction. We also demonstrate that Mims suppression effects can be used in concert with orientation-selection to edit complex ^[1,2]H ENDOR patterns that can be produced by even a 'simple' center with a single Mn(II). We develop a perturbation-based approach to understanding second-order shifts in Mn

SUBMITTER: Doan PE 

PROVIDER: S-EPMC12326263 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature