Kinetic Modeling of the Reversible or Irreversible Electrochemical Responses of FeFe-Hydrogenases.

ABSTRACT: The enzyme FeFe-hydrogenase catalyzes H₂ evolution and oxidation at an active site that consists of a [4Fe-4S] cluster bridged to a [Fe₂(CO)₃(CN)₂(azadithiolate)] subsite. Previous investigations of its mechanism were mostly conducted on a few "prototypical" FeFe-hydrogenases, such as that from Chlamydomonas reinhardtii(Cr HydA1), but atypical hydrogenases have recently been characterized in an effort to explore the diversity of this class of enzymes. We aim at understanding why prototypical hydrogenases are active in either direction of the reaction in response to a small deviation from equilibrium, whereas the homologous enzyme from Thermoanaerobacter mathranii (Tam HydS) shows activity only under conditions of very high driving f

SUBMITTER: Fasano A 

PROVIDER: S-EPMC12326359 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature