Physicochemical Comparison of Kolliphor HS 15, ELP, and Conventional Surfactants for Antibody Stabilization in Biopharmaceutical Formulations.

ABSTRACT: Stabilization of therapeutic protein formulations with nonionic surfactants such as Polysorbate 20 (PS20), Polysorbate 80 (PS80), or Poloxamer 188 (P188) is imperative to avoid critical loss of the active pharmaceutical ingredient by aggregation or adsorption onto different types of interfaces. In the present work, we have characterized the interfacial activity of the aforementioned surfactants, alone and in competition with antibodies, in comparison to two other excipients with approval for use in parenteral applications, Kolliphor HS 15 (HS15) and Kolliphor ELP (ELP). To this end, we applied a comprehensive suite of experimental techniques, including tensiometry, interfacial rheology, and quartz-crystal microbalance with dissipation monitoring (QCM-D). The obtained data shows important d

SUBMITTER: Low N 

PROVIDER: S-EPMC12326363 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature