Single-cell transcriptome sequencing reveals the sphingolipid metabolism pathway plays an important role in leaf senescence in tobacco.

ABSTRACT: Tobacco was an important cash crop and model plant, and the senescence (yellowing) of its leaves were essential markers of harvesting and secondary metabolite formation. However, the cellular senescence process and its regulatory mechanisms in tobacco leaves remain unknown. Therefore, we constructed single-cell transcriptome profiles using tobacco leaf tissues at the maturation and senescence stages to clarify the molecular mechanism of the process. We obtained 17,100 high-quality cells and sorted them into 15 clusters. Based on the marker genes of the leaf cells, we sorted the 15 clusters into 10 cell types, including palisade mesophyll, spongy mesophyll, epidermis, xylem, phloem, hydathode, companion, bundle sheath, guard, and trichome cells. Comparison of gene expression patterns in mat

SUBMITTER: Sun Q 

PROVIDER: S-EPMC12326597 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature