The role of cervical microbiome in cervical incompetence: insights from 16 S rRNA metagenomic sequencing.

ABSTRACT: Cervical incompetence (CI) is recognized as a critical factor contributing to mid-pregnancy miscarriage and preterm delivery, significantly affecting pregnancy outcomes. Despite this, the specific role of the microbiome in this pathological process remains inadequately understood. This study seeks to elucidate the core microbiome associated with CI in pregnant women and explore its potential biological mechanisms. Utilizing 16 S rRNA metagenomic sequencing, we examined the cervical mucus microbiota of women with CI both pre-operatively (PreOp) and post-operatively (PostOp). Subsequently, the immunomodulatory effects of these microbial communities on the immune system were systematically assessed using quantitative real-time PCR, Western blotting, and enzyme-linked immunosorbent assay. The

SUBMITTER: Jingwen J 

PROVIDER: S-EPMC12326704 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature