Triptolide targets PPP2CA/ITGA5 axis to suppress lactate-driven ovarian cancer progression.

ABSTRACT:

Background

Triptolide, the active compound of Tripterygium wilfordii, exhibits broad anti-tumor activity. This study explores PPP2CA dysregulation in ovarian cancer (OC) progression via lactate production and evaluates Triptolide's potential to regulate this process.

Methods

We used patient-derived xenograft (PDX) models, cell proliferation, and migration assays to assess lactate's impact on OC progression. CRISPR-Cas9 was applied to knock out PPP2CA, examining its effect on lactate production and tumor progression. RNA-seq analyzed transcriptomic changes post-PPP2CA knockout. The PPP2CA-ITGA5 axis was validated using xenografts, immunofluorescence, immunohistochemistry staining and western blot. Exosome isolation and co-culture experiments with tumor cells and human periton

SUBMITTER: Ding L 

PROVIDER: S-EPMC12326723 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature