Identification of replicative aging and inflammatory aging signatures via whole-genome CRISPRi screens.

ABSTRACT:

Background

Aging is a major risk factor for chronic diseases and cancer. Cellular aging, particularly in adult stem cells, offers a high-throughput framework for dissecting the molecular mechanisms of aging.

Results

We perform multiple genome-wide CRISPR interference (CRISPRi) screenings in human primary mesenchymal stem cells derived from adipose tissue during either replicative senescence or inflammation-induced senescence. These screens reveal distinct sets of potential novel regulators specific to each senescence pathway. Combining our perturbation-based functional genomic data with 405 genome-wide association study datasets, including 50 aging-related studies, we find that the inflammatory aging signatures identified from CRISPRi screenings are significantly associated

SUBMITTER: Wu L 

PROVIDER: S-EPMC12326738 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature