Enhancement of carrimycin production in Streptomyces spiramyceticus 54-IA by the reporter-guided selection method with traditional mutagenesis.

ABSTRACT: Carrimycin, a new drug approved in recent years, is produced by recombinant Streptomyces spiramyceticus harboring a heterogeneous 4″-O-isovaleryltransferase gene (ist). The regulatory gene bsm42 plays a crucial role in activation of carrimycin biosynthesis, its high expression can improve the carrimycin production. The xylE-neo reporter cassette under control of the the two different lengths of bsm42 promoters, F1R and F2R, can be used to monitor the expression of bsm42 to screen carrimycin high-yield mutants treated with the composite mutagenesis of plasma and ultraviolet radiation. We found that 47% of the mutants selected based on the higher expression of reporter genes driven by bsm42 promoters showed improved carrimycin production, and the shorter promoter F2R was more effective than

SUBMITTER: Li K 

PROVIDER: S-EPMC12326811 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature