STIM2β is a Ca²⁺ signaling modulator for the regulation of mitotic clonal expansion and PPARG2 transcription in adipogenesis.

ABSTRACT: Intracellular Ca²⁺ is crucial in the regulation of adipocyte lipid metabolism and adipogenesis. In this study, we aimed to investigate the regulation mechanism of intracellular Ca²⁺ levels ([Ca²⁺]_i) during adipocyte differentiation. We found that the expression of stromal interaction molecule 2 beta (STIM2β), which is the inhibitor of store-operated Ca²⁺ entry (SOCE), is upregulated throughout the differentiation process. Evaluation of [Ca²⁺]_i in 3 T3-L1 and primary stromal vascular fraction (SVF) cells revealed that the basal Ca²⁺ level is downregulated after differentiation. Knockout (KO) of STIM2β in 3T3-L1 and primary SVF cells showed increased [Ca²⁺]_i, indicating the involveme

SUBMITTER: Jeong SJ 

PROVIDER: S-EPMC12326919 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature