Active site loops of membrane-anchored metallo-β-lactamases from environmental bacteria determine cephalosporinase activity.

ABSTRACT: Antimicrobial resistance is a significant global public health threat that limits treatment options for bacterial infections. This situation is aggravated by the environmental spread of β-lactamase genes. In particular, metallo-β-lactamases (MBLs) hydrolyze almost all available β-lactam antibiotics, including late-generation cephalosporins and carbapenems. Among MBLs, the New Delhi metallo-β-lactamase (NDM-1) of subclass B1 has shown the most ominous dissemination. NDM variants are the only MBLs of clinical importance that are membrane-anchored, a sub-cellular localization that endows them with high stability under conditions of metal limitation. However, antibiotic resistance predates modern antibiotic usage, and environmental bacteria serve as reservoirs for resistance genes. Here, we re

SUBMITTER: Carnevale MC 

PROVIDER: S-EPMC12326980 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature