Identification of novel compounds with prophylactic activity against hypnozoites using a <i>Plasmodium cynomolgi in vitro</i> model.

ABSTRACT: This study aimed to identify new compounds with prophylactic activity against Plasmodium liver stages, particularly hypnozoites. A small, focused set of 568 compounds from the GSK open-access TCAMS library was tested in vitro against Plasmodium cynomolgi liver stages. Among these compounds, 12 showed IC₅₀ values below 2 µM against hypnozoites, with the most active compound displaying nanomolar activity. These active compounds could be assigned to/grouped into six chemical clusters. Some compounds could be associated with potential known modes of action such as cytochrome bc1 inhibition or kinase inhibition, whereas the mode of action of other hit compounds is currently unknown. The high hit rate in this screening may be attributed to preselecting compounds b

SUBMITTER: Zeeman A-M 

PROVIDER: S-EPMC12327006 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature