Natural Deep Eutectic Solvent-Dipotassium Phosphate Aqueous Two-Phase Systems: Physicochemical Characterization, Selective Partitioning of Amino Acids and Glucose, and Functional Insight into Maillard Reaction Applications.

ABSTRACT: Despite the growing interest in natural deep eutectic solvents (NADESs) for green separation, critical aspects of their structural stability in aqueous two-phase systems (ATPS), solute partitioning mechanisms, and potential as reaction media remain poorly understood. This study investigates the development and application of NADES-K₂HPO₄ ATPS. Four NADES formulations, namely, betaine-glycerol (Bet:Gly), betaine-propylene glycol (Bet:PG), choline chloride-glycerol (ChCl:Gly), and choline chloride-propylene glycol (ChCl:PG), were synthesized and characterized using ¹H NMR and differential scanning calorimetry (DSC). The phase-forming ability of the NADES-K₂HPO₄ ATPS was influenced by the hydrophobicity of the NADES; specifically, the Bet

SUBMITTER: Chen K 

PROVIDER: S-EPMC12327354 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature