Lineage trajectories and fate determinants of postnatal neural stem cells and ependymal cells in the developing ventricular zone.

ABSTRACT: The ventricular zone (VZ) harbors the largest neurogenic niche in the adult mammalian brain and is consisted of neural stem cells (NSCs) and multiciliated ependymal cells (EPCs). Previous lineage tracing studies showed that both NSCs and EPCs were derived from radial glial cells (RGCs). However, the transcriptomic dynamics and the molecular mechanisms guiding the cell fate commitment during the differentiation remain poorly understood. In this study, we analyzed the developing VZ of mice at single-cell resolution and identified three distinct cellular states of RGCs: bipotent glial progenitor cells (bGPCs), neonatal NSC-neuroblasts (nNSC-NBs) and neonatal EPCs (nEPCs). The differentiation from bGPCs to nNSC-NBs and nEPCs forms a continuous bifurcating trajectory. Analysis along the NSC bra

SUBMITTER: Zheng J 

PROVIDER: S-EPMC12327645 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature