Cellular Reprogramming by PHF7 Enhances Cardiac Function Following Myocardial Infarction.

ABSTRACT:

Background

Direct reprogramming of fibroblasts to cardiomyocytes is a potentially curative strategy for ischemic heart disease. However, current reprogramming strategies require excessive factors due to epigenetic barriers of adult mouse and human fibroblasts. Recently, we identified the epigenetic factor PHF7 from a screen of gene-regulatory factors as the most potent activator of adult fibroblast-to-cardiomyocyte reprogramming in vitro.

Methods

Through in vitro assays coupled with genome-wide studies, we interrogated the ability of PHF7 to induce reprogramming events with minimal reprogramming factors. Using in vivo murine models of myocardial infarction and intramyocardial reprogramming factor delivery coupled with genetic fibroblast lineage tracing, we delivered retrovir

SUBMITTER: Bann GG 

PROVIDER: S-EPMC12327843 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature