Spatial transcriptomics reveals human cortical layer and area specification.

ABSTRACT: The human cerebral cortex is composed of six layers and dozens of areas that are molecularly and structurally distinct^1-4. Although single-cell transcriptomic studies have advanced the molecular characterization of human cortical development, a substantial gap exists owing to the loss of spatial context during cell dissociation^5-8. Here we used multiplexed error-robust fluorescence in situ hybridization (MERFISH)⁹, augmented with deep-learning-based nucleus segmentation, to examine the molecular, cellular and cytoarchitectural development of the human fetal cortex with spatially resolved single-cell resolution. Our extensive spatial atlas, encompassing more than 18 million single cells, spans eight cortical areas across seven developmental time points. We u

SUBMITTER: Qian X 

PROVIDER: S-EPMC12328223 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature