Integral membrane proteins of the chloroplast envelope: identification and subcellular localization of new transporters.

ABSTRACT: A two-membrane system, or envelope, surrounds plastids. Because of the integration of chloroplast metabolism within the plant cell, the envelope is the site of many specific transport activities. However, only a few proteins involved in the processes of transport across the chloroplast envelope have been identified already at the molecular level. To discover new envelope transporters, we developed a subcellular proteomic approach, which is aimed to identify the most hydrophobic envelope proteins. This strategy combined the use of highly purified and characterized membrane fractions, extraction of the hydrophobic proteins with organic solvents, SDS/PAGE separation, and tandem mass spectrometry analysis. To process the large amount of MS/MS data, a blast-based program was developed for searc

SUBMITTER: Ferro M 

PROVIDER: S-EPMC123283 | biostudies-literature | 2002 Aug

REPOSITORIES: biostudies-literature