Filamentation-driven peripheral clustering of the inducible lysine decarboxylase is crucial for E. coli acid stress response.

ABSTRACT: Bacteria use sophisticated acid stress response strategies to withstand fluctuating environmental pH, with enterobacterial inducible amino acid decarboxylases playing a major role. The lysine decarboxylase LdcI catalyses lysine-to-cadaverine conversion coupled to proton consumption and carbon dioxide release, thereby buffering cytoplasmic and extracellular pH. Our previous studies showed that Escherichia coli LdcI forms intracellular patches under mild acid stress, and that purified LdcI polymerises into filaments at acidic pH. Here, we investigated the physiological relevance of LdcI filamentation using 3D super-resolution microscopy and an LdcI polymerisation-deficient E. coli mutant strain. We established a semi-automated workflow for intracellular cluster detection and quantitative ana

SUBMITTER: Kirchner MA 

PROVIDER: S-EPMC12328647 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature