ALDH18A1 has carcinogenic functions and regulates alternative splicing events of DNA repair-related genes in esophageal carcinoma cells.

ABSTRACT: Esophageal carcinoma (ESCA) is a common tumor in the digestive system, resulting in approximately 300,000 death worldwide every year. ALDH18A1, a potential RNA binding protein (RBP), is significantly overexpressed as in ESCA, while its functions and mechanism is unclear. In this study, we silenced ALDH18A1 in KYSE150 cells using small interfering RNA (siALDH18A1), and evaluated its effect on cell characteristics. Finally, we conducted whole transcriptome sequencing (RNA-seq) and RNA immunoprecipitation (iRIP-seq) experiments to explore the downstream targets of ALDH18A1, and verified their change patterns using quantitative polymerase chain reaction. We found that ALDH18A1 exhibited a high expression level in ESCA patients using online database. The proliferation, invasion, and migration a

SUBMITTER: Yongkang W 

PROVIDER: S-EPMC12328819 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature