Functional study of Phaeodactylum tricornutum Seipin highlights specificities of lipid droplets biogenesis in diatoms.

ABSTRACT: Diatoms are a major phylum of microalgae, playing crucial ecological roles. They derive from secondary endosymbiosis, leading to a complex intracellular architecture. Their ability to store oil in lipid droplets (LDs) upon unfavourable conditions has raised interest for applications, in particular biofuels, yet Lipid Droplet (LD) biogenesis mechanisms in these organisms remain poorly understood. Here, we functionally characterize the homolog of Seipin, a major actor of LD biogenesis, in Phaeodactylum tricornutum. We used an in silico approach to analyze the evolutionary origin of PtSeipin and its specific features. Then, we used a functional genetics approach with a combination of confocal and electronic microscopy and lipidomics to characterize the protein function. We provide evidence th

SUBMITTER: Le Moigne D 

PROVIDER: S-EPMC12329175 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature