Unveiling Small Non-Coding RNA Dynamics During Recombinant Adeno-Associated Virus Production.

ABSTRACT: Recombinant adeno-associated viruses (rAAVs) play a pivotal role in gene therapy, yet the molecular interactions underlying rAAV production in host cells remain incompletely understood. Non-coding RNAs (ncRNAs), particularly microRNAs (miRNAs) and small nucleolar RNAs (snoRNAs), are increasingly recognized as key regulators of viral and cellular processes. This study investigates the dynamic expression profiles of miRNAs and snoRNAs during rAAV plasmid transfection and vector production in HEK293F cells. A total of 142 miRNAs were differentially expressed during the peak phase of rAAV production, with 128 associated with the Gene Ontology term "viral process", indicating broad involvement in host-virus interactions. Target gene analysis linked these miRNAs to biological pathways such as nu

SUBMITTER: Burkhart M 

PROVIDER: S-EPMC12329270 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature