Membrane adsorber design for lentiviral vector recovery.

ABSTRACT: The growing demand for lentiviral vectors (LVs) in cell and gene therapies has highlighted significant challenges in large-scale LV production, particularly low product recovery. Anion-exchange chromatography (AIEX) is widely used for LV capture. However, AIEX accounts for most of the product loss in downstream processing, due to excessive interaction strengths in current commercially available membranes causing irreversible LV binding. To address this, a new AIEX membrane structure that reduces LV interaction strength by lowering ligand density, using direct ligand grafting, and incorporating tighter pore size distributions is tested. Four prototypes were created with Q and D anion-exchange chemistries at 1.05 (R1) and 1.32 (R2) μm pore sizes. Prototypes minimized product loss from irreve

SUBMITTER: Pamenter G 

PROVIDER: S-EPMC12329310 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature