5-Hydroxymethylcytosine signatures in cell-free DNA as biomarkers for distinguishing acute coronary syndrome following COVID-19 infection and its association with neutrophil activation and PDE4D expression.

ABSTRACT:

Background

While 5hmC features in cell-free DNA (cfDNA) show promise as early biomarkers for COVID-19 severity, myocardial injury, and long-term outcomes, their specific role in acute coronary syndrome (ACS) triggered shortly after COVID-19 infection remains unclear.

Methods

We generated genome-wide 5hmC profiles from plasma cfDNA using the 5hmC-Seal technique across three cohorts: ACS patients without prior COVID-19 infection (ACS, n = 16), patients experiencing ACS within 2 months post-COVID-19 (ACS2N, n = 24), and patients experiencing ACS beyond 2 months post-COVID-19 (ACS2W, n = 28). Differential 5hmC analysis, functional enrichment (GO, KEGG), immune cell deconvolution, and protein-protein interaction (PPI) network analysis were performed.

Results

Significant di

SUBMITTER: Li Z 

PROVIDER: S-EPMC12329312 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature