MicroRNA analysis of porcine muscle tissue involved in phosphoinositol metabolism.

ABSTRACT:

Introduction

To elucidate the role of miRNAs in porcine muscle tissues, this study used Solexa high-throughput sequencing to identify and compare differentially expressed miRNAs in Landrace pigs and the Diannan small ear pigs.

Methods

Small RNA libraries were constructed from high-quality RNAs extracted from the Longissimus dorsi muscle (LDM) muscles of the two breeds of pigs and high-throughput sequencing was performed to identify and compare differentially expressed of miRNAs (DEmiRNAs). The target genes corresponding to the differentially expressed miRNAs were subjected to functional annotation, Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genomes (KEGG) pathway enrichment analysis.

Results

241 known conserved miRNAs sequences and 20 novel miRNA

SUBMITTER: Xie Y 

PROVIDER: S-EPMC12329466 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature