Affordable Non-Invasive Machine-Aided Phenotyping Identifies Phenotypic Variation to Soil Stress Across the Arabidopsis thaliana Life Cycle.

ABSTRACT: Arabidopsis thaliana is a model species for uncovering genetic adaptation to alkaline calcareous soils (ACS). This species thrives in ACS, often occurring in dry marginal and urban environments. Existing research largely focused on vegetatively grown seedlings, with a notable lack of studies examining phenotypic variations across the life cycle. A valuable tool for understanding stress resilience is machine-aided phenotyping, as it is non-invasive, rapid, and accurate, but often unavailable to small plant labs. Here, we established and validated an affordable multispectral machine-aided phenotyping approach implementable by individual labs. We collected and correlated quantitative growth data across the entire plant life cycle in response to ACS. We used an A. thaliana wildtype and the cou

SUBMITTER: Knopf MC 

PROVIDER: S-EPMC12329706 | biostudies-literature | 2025 Jul-Aug

REPOSITORIES: biostudies-literature