<i>Pseudomonas aeruginosa</i> Cryptic Prophage Endolysin Is a Highly Active Muramidase.

ABSTRACT: Endolysins are phage-encoded enzymes that cleave the peptidoglycan of host bacteria. These enzymes have gained considerable attention due to their ability to cause cell lysis, making them candidates as antibacterial agents. Most Pseudomonas aeruginosa genomes, including the common laboratory strains PAO1 and UCBPP-PA14, contain a cryptic prophage encoding a glycoside hydrolase family 19 endolysin (named PaGH19Lys in the present study). Family 19 glycoside hydrolases are known to target peptidoglycan and chitin-type substrates. PaGH19Lys was not active toward chitin but exhibited activity toward chloroform-treated Gram-negative bacteria, displaying ∼10,000-fold higher activity than hen egg white lysozyme. Analysis of products derived from PaGH19Lys activit

SUBMITTER: Thoren Edvardsen PK 

PROVIDER: S-EPMC12329726 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature