Association of chaperone-mediated autophagy with the mechanisms of vascular calcification in diabetic nephropathy.

ABSTRACT: Background: Vascular calcification and autophagy play pivotal roles in the pathogenesis of diabetic nephropathy (DN), though the underlying molecular mechanisms remain unclear. Methods: Differential expression analysis and weighted gene co-expression network analysis were performed on the GSE30529 dataset to identify candidate genes associated with DN. Subsequently, Mendelian randomization analysis was utilized to isolate genes with a causal relationship to DN. DN biomarkers were further validated based on their expression profiles in both the GSE30529 training set and the GSE96804 validation set. Gene set enrichment analysis, immune infiltration analysis, drug prediction, and molecular regulatory network construction were then conducted. Reverse transcription-quantitative PC

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC12329838 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature