Unravelling Artemisia species genetic variation via DNA barcoding, ISSR and RAPD with the development of eco-specific SCAR markers.

ABSTRACT:

Background

Genus Artemisia is one of the largest and most globally spread genera, comprising more than 500 species known for their phytochemical diversity and therapeutic properties. This necessitates the accurate authentication and differentiation of its species. Traditional morphological, microscopical and metabolic profiling methods are often insufficient for reliable discrimination. The aim of this study is the authentication and assessment of the genetic diversity of wild Egyptian Artemisia species; A. herba-alba, A. monosperma, A. judaica and cultivated A. annua using a combined molecular approach of DNA barcoding, ISSR, RAPD, and the development of eco-specific SCAR markers.

Results

DNA barcoding targeting both nuclear (ITS2) and plastid (psbA-trnH) spacers revealed t

SUBMITTER: Mahgoub YA 

PROVIDER: S-EPMC12329950 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature