The MADS-box gene CmAP1 promotes flowering and petal development in Chinese chestnut (Castanea mollissima).

ABSTRACT:

Background

Chinese chestnut (Castanea mollissima) is an economically and ecologically important woody nut crop. In C. mollissima, flowering is fundamental for nut yield. The MADS-box gene APETALA 1 (AP1) plays essential roles in floral initiation and floral organ development in many plants. However, the function of the AP1 gene CmAP1 in C. mollissima is still unclear. Here, we cloned the coding sequence (CDS) and promoter of CmAP1 and analyzed the function of this gene.

Results

The CDS of CmAP1 is 741 bp and encodes a 246-amino acid protein. Subcellular analysis revealed that CmAP1 localizes to the nucleus. GUS driven by the CmAP1 promoter was expressed in seedlings and in leaf margins, petals, and carpels of transgenic Arabidopsis (Arabidopsis thaliana). RNA in situ hybridi

SUBMITTER: Cheng Y 

PROVIDER: S-EPMC12330143 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature