Integrated Respirometry and Metabolomics Unveil Circadian Metabolic Dynamics in Drosophila.

ABSTRACT: Precise temporal regulation of metabolism by sleep and circadian rhythms is essential for dynamic energy homeostasis, yet the link between systemic metabolism and respiratory demands remains poorly defined. We combined high-resolution respirometry with LC-MS-based metabolomics to characterize respiratory dynamics and metabolic states in Drosophila melanogaster to uncover genotype-specific impacts of sleep and circadian disruption. Wild-type flies under light-dark cycles (WT-LD) showed rhythmic respiratory patterns reflective of anticipatory coordination of mitochondrial energy metabolism, amino acid turnover, and redox cycling. In contrast, short-sleep mutants (fmn, sss) exhibited elevated metabolic rates and reactive shifts of fuel preferences toward lipid and amino a

SUBMITTER: Akhtar F 

PROVIDER: S-EPMC12330479 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature