Chemogenetic and optogenetic strategies for spatiotemporal control of split-enzyme-based calcium recording.

ABSTRACT: Methods for monitoring physiological changes in cellular Ca²⁺ levels have been in high demand for their utility in monitoring neuronal signaling. Recently, we introduced SCANR (Split-Tobacco Etch Virus (TEV) protease Calcium-regulated Neuron Recorder), which reports on Ca²⁺ changes in cells through the binding of calmodulin and M13 to reconstitute an active TEV protease. First-generation SCANR marked all of the Ca²⁺ spikes that occur throughout the lifetime of the cell, but it did not have a mechanism for controlling the time window in which recording of physiological changes in Ca²⁺ occurred. Here, we explore both chemical and light-based strategies for controlling the time and place in which Ca²⁺ recording occurs. We describe the ada

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC12330574 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature