Combined Deletion of miR-27a and miR-27b Enhances Protection Against Diet-Induced Obesity.

ABSTRACT: Obesity impairs adipose tissue thermogenesis, contributing to metabolic dysfunction. Here, we identify miR-27a and miR-27b as cooperative regulators of adipose tissue thermogenesis and adipogenic programming in the context of diet-induced obesity (DIO). Intervention of high-fat diet (HFD) to mice suppressed the expression of Ucp1, Ppary, and Pgc1a in inguinal white adipose tissue (WAT), which correlated with the increased expression of miR-27a/b. Using global knockout models for miR-27a and miR-27b, we identified that combined deletion of both miRNAs (double knockout, DKO) synergistically enhances Ucp1 expression, mitochondrial heat production and browning of WAT. DKO mice displayed improved glucose, insulin sensitivity and reduced adiposity under HFD conditions and ou

SUBMITTER: Kesharwani D 

PROVIDER: S-EPMC12330670 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature