A Supramolecular Self-assembly Approach to Site-Specific Antibody Conjugates <i>via</i> a Coiled-coil Peptides Platform.

ABSTRACT: Antibody conjugates play a central role across multiple healthcare sectors with a prime example being antibody-drug conjugates (ADCs). Although widely used lysine and hinge cysteine conjugation methods yield products, the lack of site-specificity and spatial control along with the highly heterogeneous composition are significant limitations. We describe a facile supramolecular assembly method based on heterodimer coiled-coil formation for site-specific antibody conjugation. The method affords uniform loading of diverse payloads including anti-cancer agents, polymers, enzymes, fluorophores, etc. under mild aqueous conditions. Further, the facile convergent approach capitalizes on the independent strengths and flexibility of protein expression and peptide chemistry culminating in a final sel

SUBMITTER: Ringaci A 

PROVIDER: S-EPMC12330717 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature