Human iPSC derived alveolar macrophages reveal macrophage subtype specific functions of itaconate in <i>M. tuberculosis</i> host defense.

ABSTRACT: Mycobacterium tuberculosis (Mtb) must survive within multiple macrophage populations during infection, including alveolar macrophages (AM) and recruited inflammatory macrophages. In mice, itaconate, produced in macrophages by ACOD1 mediated decarboxylation of aconitate, has direct antimicrobial activity, modulates inflammatory cytokines, and is required for resistance to M. tuberculosis (Mtb) infection. The role of itaconate in human macrophages is less clear and whether itaconate mediates distinct effects in macrophage subtypes is unknown. Here, we investigated the role of itaconate in human iPSC-derived macrophages, either induced by GM-CSF to resemble alveolar macrophages (AM-Like cells), or treated with M-CSF to generate control macrophages (MCDM cells). Both types of hum

SUBMITTER: Krebs A 

PROVIDER: S-EPMC12330731 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature