Actin Filament Barbed-End Depolymerization by Combined Action of Profilin, Cofilin, and Twinfilin.

ABSTRACT: Cellular actin dynamics result from collective action of hundreds of regulatory proteins, majority of which target actin filaments at their barbed ends. Three key actin binding proteins - profilin, cofilin and twinfilin individually depolymerize filament barbed ends. Notwithstanding recent leaps in our understanding of their individual action, how they collectively regulate filament dynamics remains an open question. In absence of direct and simultaneous visualization of these proteins at barbed ends, gaining mechanistic insights has been challenging. We have here investigated multicomponent dynamics of profilin, cofilin and twinfilin using a hybrid approach that combines high throughput single filament experiments with theory. We discovered that while twinfilin competes with profilin, it

SUBMITTER: Arya A 

PROVIDER: S-EPMC12330814 | biostudies-literature | 2024 Jul-Sep

REPOSITORIES: biostudies-literature