SynchroSep-MS: Parallel LC Separations for Multiplexed Proteomics.

ABSTRACT: Achieving high throughput remains a challenge in MS-based proteomics for large-scale applications. We introduce SynchroSep-MS, a novel method for parallelized, label-free proteome analysis that leverages the rapid acquisition speed of modern mass spectrometers. This approach employs multiple liquid chromatography columns, each with an independent sample, simultaneously introduced into a single mass spectrometer inlet. A precisely controlled retention time offset between sample injections creates distinct elution profiles, facilitating unambiguous analyte assignment. We modified the DIA-NN workflow to effectively process these unique parallelized data, accounting for retention time offsets. Using a dual-column setup with mouse brain peptides, SynchroSep-MS detected approximately 16,700 uniq

SUBMITTER: Lancaster NM 

PROVIDER: S-EPMC12331150 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature