CXCL16 Producing Tumor Clones Are Shaping Immunosuppressive Microenvironment in Squamous Cell Carcinoma via CXCR6 Regulatory T Cell.

ABSTRACT:

Background

Cutaneous squamous cell carcinoma (cSCC) and basal cell carcinoma (BCC) are the most prevalent types of nonmelanoma skin cancer (NMSC) and exhibit significant inter- and intra-tumor heterogeneity. cSCC has a higher metastatic potential than BCC, accompanied by a considerable mortality rate. However, the detailed mechanisms of tumor evolution in cSCC have not yet been described.

Methods

We performed single-cell RNA sequencing (scRNA-seq) and T cell receptor (TCR) clonal analysis of skin biopsies from five BCCs, three squamous cell carcinomas in situ (SCCIS), and two invasive squamous cell carcinomas (SCC). Independent SCC specimens were used for spatial transcriptomic (ST) analysis using GeoMx Digital Spatial Profiler (DSP).

Result

Using scRNA-seq, we analyz

SUBMITTER: Choi HS 

PROVIDER: S-EPMC12331524 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature