Determination of longitudinal Circulating levels of miR-21-5p, miR-23b-3p and miR-34a-5p in plasma of patients with glioblastoma using droplet digital PCR.

ABSTRACT: Glioblastoma (GBM) is characterized by molecular heterogeneity and rapid progression. Liquid biopsy is increasingly recognised as a promising non-invasive biomarker for GBM diagnosis, prognosis and treatment monitoring. The aim of the study was to explore circulating microRNAs (miRNA) as potential GBM-biomarkers. We developed droplet digital PCR (ddPCR) assays to quantify plasmatic concentrations of miR-21-5p, miR-23b-3p and miR-34a-5p both in healthy volunteers and GBM patients at diagnosis and over follow-up (1, 3, 6 and 12 months after surgery). Furthermore, we investigated the correlations between peripheral miRNA levels and clinical, neuroradiological, pathological features, extent of tumour resection, overall (OS) and recurrence-free (RFS) survivals. Our findings showed that these mi

SUBMITTER: Donofrio CA 

PROVIDER: S-EPMC12331880 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature