Multi-photon, label-free photoacoustic and optical imaging of NADH in brain cells.

ABSTRACT: Label-free detection of biological events at single-cell resolution in the brain can non-invasively capture brain status for medical diagnosis and basic neuroscience research. NADH is an universal coenzyme that not only plays a central role in cellular metabolism but may also be used as a biomarker to capture metabolic processes in brain cells and structures. We have developed a new label-free, multiphoton photoacoustic microscope (LF-MP-PAM) with a near-infrared femtosecond laser to observe endogenous NAD(P)H in living cells. The imaging depth of NAD(P)H in tissues with all-optical methods is limited to ~100 μm in brain tissue by the strong absorption of the near-ultraviolet fluorescence. Here, acoustic detection of the thermal signature of multi-photon (three-photon) excitation of NAD(P)

SUBMITTER: Osaki T 

PROVIDER: S-EPMC12331929 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature