Conditional guide RNA deactivation by mRNA and small molecule triggers in Saccharomyces cerevisiae.

ABSTRACT: CRISPR interference (CRISPRi) technologies have revolutionized bioengineering by providing precise tools for gene expression modulation, enabling targeted gene perturbation and metabolic pathway optimization. Despite these advances, achieving dynamic control over gene expression by CRISPR-based regulation remains a challenge due to its inherently static nature. Utilizing toehold-mediated strand displacement and ligand-responsive ribozymes (aptazymes), this study introduces switchable guide RNAs (gRNAs) that facilitate tunable gene expression mediated by mRNA or small molecule signals. We demonstrate complete silencing of gRNA via strategically designed 5' or 3' extensions that impede the gRNA spacer or the dCas9 handle, with subsequent restoration of function through sequestration or cleav

SUBMITTER: Xi C 

PROVIDER: S-EPMC12332140 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature