Functional Characterization of Polyketide Synthase Clusters in Streptomyces anandii J6.

ABSTRACT: Streptomyces species are well-known for their antifungal properties and the production of diverse secondary metabolites, including non-ribosomal peptides and polyketides. These metabolites can be identified through various genetic techniques, allowing for the investigation of gene functions using whole-genome databases. Numerous studies have explored the genetic functions of Streptomyces using advanced techniques, such as CRISPR-Cas9 mutagenesis, to generate site-specific mutant strains. In this study, we re-identified Streptomyces sp. J6 as Streptomyces anandii J6 through whole-genome sequencing and average nucleotide identity (ANI) analysis. The type II and type III polyketide synthase clusters (PKS: clusters 9, 10, and 12) were further studied using CRISPR-Cas9 for functional analysis,

SUBMITTER: Kim DR 

PROVIDER: S-EPMC12332411 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature