Evaluation of Colistin Susceptibility in Carbapenem-Resistant Acinetobacter baumannii Isolates Using Broth Microdilution, MICRONAUT-MIC-Strip, and VITEK MS.

ABSTRACT: We aimed to analyze the colistin susceptibility using broth microdilution (BMD) and commercially available MICRONAUT-MIC-Strip (MMS) test and VITEK MS in carbapenem-resistant Acinetobacter baumannii (CRAB) isolates. Colistin susceptibility of 194 CRAB isolated from various clinical specimens between December 2020 and 2022 was determined by BMD and commercial MMS method. Minimum inhibitory concentrations of the commercial method were compared with the standard BMD. In the second part of the study, colistin susceptibility of the isolates was tested using the MALDIxin method, which detects modified lipid A, by VITEK MS, MALDI-TOF MS (bioMérieux, France). The presence of mcr-1-5 genes in resistant isolates was investigated using in-house multiplex polymerase chain reaction. Of these, 23 (11.8%

SUBMITTER: Akıllı FM 

PROVIDER: S-EPMC12332535 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature