Self-Assembling Aromatic Peptide Amphiphile Fibers for Multivalent Display of Enzymatically Linked Antigenic Proteins.

ABSTRACT: Supramolecular fibers assembled from peptide amphiphiles are promising materials for the delivery of biopharmaceuticals. However, strategies for directly conjugating folded proteins onto these supramolecular dynamic assemblies remain limited. Herein, we demonstrate that aromatic peptide amphiphiles that integrate self-assembly motifs with enzymatic recognition sequences enable the synthesis of supramolecular fibrous materials amenable to protein conjugation in their native folded state. The designed peptide amphiphiles self-assembled into fibers through a combination of hydrophobic, aromatic, and hydrogen bonding interactions in aqueous media. Using microbial transglutaminase, a recombinant enhanced green fluorescent protein (EGFP), used as a model proteinaceous antigen, was covalently cou

SUBMITTER: Wakabayashi R 

PROVIDER: S-EPMC12332845 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature