A Chemically Stable Photocaged Noradrenaline.

ABSTRACT: Photoactivatable neurotransmitters provide spatiotemporally precise experimenter control over endogenous receptor activation in living tissue. The resulting optical stimulus-neuronal response relationship provides a sensitive assay that can drive quantitative studies into receptor signaling. Here, we report a photocaged derivative of the prominent catecholamine neurotransmitter noradrenaline (NA). Appending a carboxynitroveratryl (CNV) caging group to the 4-hydroxyl of the catechol group produced CNV-NA, which displays good aqueous solubility and chemical stability. We verified CNV-NA's lack of activity at α_1B- and β₂-adrenoreceptors expressed in HEK cells using a live-cell cAMP assay. We validated CNV-NA photoactivation at native α₂-adrenoreceptors in brai

SUBMITTER: Buczynski SA 

PROVIDER: S-EPMC12333006 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature