Reductive Dechlorination of 2,3-Dichloroaniline by <i>Dehalobacter</i> in an Anaerobic Enrichment Culture.

ABSTRACT: 2,3-dichloroaniline (2,3-DCA) has widespread use in chemical manufacturing and remains a persistent groundwater contaminant. To better understand the pathway and kinetics of its reductive dechlorination, we conducted a laboratory kinetic experiment using an anaerobic enrichment culture dominated by Dehalobacter. At an initial field-relevant concentration of 40 mg/L, complete and stoichiometric dechlorination of 2,3-DCA to aniline via 2-chloroaniline (2-CA) was achieved. The intermediates, 2-CA and 3-chloroaniline, were transiently formed in a ratio of 8:1. The growth yields of Dehalobacter on 2,3-DCA and 2-CA were 1.2 ± 0.1 × 10⁸ and 1.3 ± 0.1 × 10⁸ 16S rRNA gene copies/μmol chloride released, respectively. The maximum specific growth rate for 2,3-DCA, μ

SUBMITTER: Wang S 

PROVIDER: S-EPMC12333009 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature