Characterization of private mutations in the spike protein of SARS-CoV-2 correlates with viral prevalence.

ABSTRACT:

Background

SARS-CoV-2 has continued to spread globally since its outbreak. As the virus evolves, new variants affect the efficacy of current vaccines and treatments. Therefore, understanding and explaining the viral prevalence of SARS-CoV-2 is crucial for developing effective public health strategies.

Methods

Genomic information from the SARS-CoV-2 network surveillance laboratories in Jiangsu Province in 2023 was collected. The impact of private mutations on binding affinity of the spike protein to Angiotensin Converting Enzyme 2 (ACE2) was evaluated by Bioinformatic analyses, pseudovirus infection assays and Bio-Layer Interferometry (BLI). The impact of private mutations on antibody escape capacity was evaluated through Pseudotyped Virus Neutralization Assay (PVNA).

Resu

SUBMITTER: Wang S 

PROVIDER: S-EPMC12333205 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature