Novel Insights into the Human Gut Microbially Conjugated Bile Acids: The New Diversity of the Amino Acid-Conjugated Derivatives.

ABSTRACT: Bile acids (BAs) are biomolecules involved in lipids and glucose metabolism, and recently discovered microbially conjugated BAs (MCBAs) play an important role. Although the production of MCBAs amidated with amino acids (AA), similarly to those hepatically produced, has been confirmed, new structural isomers, conjugated with non-proteinogenic AA, remain unidentified. This study evaluates the production of MCBAs by human gut microbiota and discriminates, for the first time, between structural isomers. Thirteen MCBAs composed of lithocholic acid conjugated either with the AA valine and leucine or the non-proteinogenic AA, 5-amino valeric acid, and different aminobutyric acid derivatives (GABA and 2-amino-butyric) were confirmed by MS/MS fragmentation patterns and authentic standards. Only the

SUBMITTER: Garcia CJ 

PROVIDER: S-EPMC12333354 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature